Service 01 · Rescue

Rescue a stalled selection.

You ran SELEX, it enriched — but nothing bound, or truncation killed your lead. Send us the sequencing pool. We re-read it under real buffer conditions and hand back the candidates actually worth making.

Turnaround
3–5 days
First look
Free
You send
FASTQ / FASTA
Your data
Stays yours · NDA
~30%
of "failed" pools rescued. On selections that Geneious Prime and ChatGPT couldn't analyze, our engine — trained on hundreds of Liu-lab pools — recovered 6 families of real aptamers. The rest simply had no binder to find; SELEX itself sometimes fails.
Sound familiar?

Enrichment isn't binding. That's the gap.

Nothing bound

The pool converged on a few high-count sequences — but the top reads don't bind, and the real binder is buried lower down.

Truncation killed it

You had a lead, minimized it, and lost the affinity — with no way to know which region actually mattered.

Hundreds of look-alikes

Full-length clustering split one binding family across dozens of clusters, so you can't tell what's really distinct.

How it works

From your pool to a shortlist, in four steps.

01

Send your data

Ship us the raw FASTQ (or a count table) and your selection buffer. NDA first if you want one.

02

We re-read it

Fold under your real Na⁺/Mg²⁺/K⁺/Ca²⁺, detect G-quadruplexes, and cluster by loop structure — not full-length sequence.

03

You get a shortlist

A ranked, structure-aware set of candidates, each with its conserved motif and where to truncate.

04

Make the winners

Synthesize the few worth testing — and skip the rounds you'd have wasted on look-alikes.

What you get

A shortlist you can act on.

Not a data dump — a decision. Every candidate comes with the evidence you need to synthesize it with confidence.

  • Ranked candidate shortlist (typically 3–8)
  • Structure under your real assay buffer, with G-quadruplexes flagged
  • The conserved motif per family — your likely binding core
  • Truncation map: where to cut, where not to
  • A one-page report, synthesis-ready (no ambiguous bases)
Sample deliverable
rescue_report.pdf
› 5 candidates ranked
› fold @ 10 mM Mg²⁺, 25 °C
› 2 G4 flagged
› truncation map per lead
› order sheet — no N / no chimeras
Pricing

Start free. Pay when it's worth it.

First look
Free

Send one pool. We return a short shortlist so you can judge the value on your own data before paying anything.

Full rescue
Contact for price

Full re-analysis, complete report, and a working call to walk through the results. Priced per pool — a fraction of one wasted synthesis round.

Questions

The obvious ones.

Who sees my sequences?

Only us, under NDA if you'd like one signed first. Your data stays yours; we don't publish or reuse it without written permission.

What do you need from me?

The raw FASTQ (or a sequence + count table) and your selection buffer composition. That's enough to start.

What if there's genuinely nothing there?

We'll tell you — honestly and early. A clear "this pool won't give you a binder" saves you months, and the first look costs nothing.

Can you also run the wet-lab validation?

Yes — that's our Discovery service. Many clients start with a Rescue, then have us run the selection or validation end-to-end.

Send us the pool you're stuck on.

Start a free first look →
AptaPilot — Rescue← All services