Buffer-realistic folding, G-quadruplex detection, family placement, and clean Kd — the structural picture your paper (or your investors) needs, on candidates you already hold.
mfold and UNAFold assume 1 M Na⁺ and no Mg²⁺ — not the divalent buffer your assay actually runs in.
Classic tools draw a stem-loop where CD says G-quadruplex — and you can't tell which structure actually does the binding.
Fitting binding curves in a spreadsheet macro is slow, fragile, and hard to drop cleanly into a figure.
Your candidate sequences and the buffer you assay in — plus a titration series if you want Kd fit.
Structure under explicit Na⁺/Mg²⁺/K⁺/Ca²⁺ and temperature, with native G-quadruplexes detected and drawn.
A strict quadratic binding model fit to your titration data, exported as a clean, figure-ready curve.
Structures, G4 calls, Kd, and where each candidate sits among its binding family — in one PDF.
The structural and binding picture your manuscript reviewers — or a due-diligence team — will ask for, generated correctly the first time.
Full structural + binding report for one sequence, including Kd fitting if you provide the titration data.
A reduced per-candidate rate for a set — a whole family, a screen, or a manuscript's worth of characterization. Contact for pricing.
The sequences and your assay buffer. For a Kd, add your concentration/signal titration data — we fit it and return the curve.
Characterize works from data you already have. If you need the wet-lab binding run itself, that's Discovery.
Both. It's included when we run a Rescue or Discovery, and available on its own for candidates from any source.
Yes — the folding and Kd tools are free for academics. Characterize is for when you'd rather have the finished, checked report.